4I46
Crystal structure of 31kD Heat Shock Protein, VcHsp31 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.287, 80.030, 133.037 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 94575 | |
| Completeness [%] | 91.1 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 5% PEG6000, 10% MPD, pH 5.0, VAPOR DIFFUSION, temperature 298K |
