4I44

Aer2 poly-HAMP domains: V33G HAMP1 inverted signaling mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	193
Detector technology	CCD
Collection date	2010-06-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97857
Spacegroup name	P 43 21 2
Unit cell lengths	113.362, 113.362, 64.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.880
Rwork	0.235
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.930
High resolution limit [Å]	2.880	7.810	2.880
Rmerge	0.040	0.023	0.349
Number of reflections	10197
<I/σ(I)>	22.3
Completeness [%]	99.4	92.3	100
Redundancy	9.4	7.7	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	1.1 M LITHIUM SULFATE, 15-18% GLYCEROL, 0.1 M TRIS, PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K