4I3N

Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant D61N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Detector technology	CCD
Collection date	2010-12-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	168.629, 168.629, 301.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.680 - 2.950
R-factor	0.22879
Rwork	0.227
R-free	0.25716
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xoa
RMSD bond length	0.006
RMSD bond angle	0.992
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.680	4.660	3.110
High resolution limit [Å]	2.950	4.170	2.950
Number of reflections	35000
Completeness [%]	100.0	100	100
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9.5		pH 9.5, EVAPORATION