4I3M
Aer2 poly-HAMP domains: L44H HAMP1 CW-lock mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97720 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 61.058, 61.058, 81.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| Rwork | 0.208 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.982 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 5.320 | 1.960 |
| Rmerge | 0.074 | 0.093 | 0.330 |
| Number of reflections | 12597 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 99.7 | 93.7 | 100 |
| Redundancy | 10.7 | 9.9 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5-1.7M magnesium sulfate, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
