4I3B
Crystal structure of fluorescent protein UnaG wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.394, 73.954, 124.364 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 31.923 - 1.199 |
| R-factor | 0.1305 |
| Rwork | 0.129 |
| R-free | 0.15860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fdq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8.1_1168) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.054 | 0.226 |
| Number of reflections | 457015 | |
| <I/σ(I)> | 35 | 1.6 |
| Completeness [%] | 80.0 | 27.5 |
| Redundancy | 2.4 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2M Ammonium acetate, 0.1M Sodium citrate tribasic dihydrate, 20%w/v Polyethylene glycol 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
