4I2Z

Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	86.633, 86.633, 815.015
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.931 - 2.900
R-factor	0.2384
Rwork	0.237
R-free	0.25590
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.440
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Number of reflections	41180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M HEPES, 10% PEG 8000, 12% ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K