4I2U
Crystal structure of the reduced glutaredoxin from Chlorella sorokiniana T-89 in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.676, 51.310, 55.658 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.463 - 1.300 |
| R-factor | 0.1416 |
| Rwork | 0.139 |
| R-free | 0.16640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.039 | 0.312 |
| Number of reflections | 24365 | |
| <I/σ(I)> | 30.615 | 2.925 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5mM DTT, 30%(w/v) PEG 3000 , 0.1M CHES, pH 9., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
