4I2T
Crystal structure of the oxidized glutaredoxin from Chlorella sorokiniana T-89
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.798, 47.186, 38.573 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 23.593 - 1.400 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7p |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.034 | 0.240 |
| Number of reflections | 23696 | |
| <I/σ(I)> | 35.528 | 3.42 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M ammonium acetate, 35% PEG 4000, 0.11M Tri-sodium dihydrate citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
