4I2I
Binary complex of mouse TdT with AP5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.750, 84.100, 103.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.100 - 2.500 |
| R-factor | 0.1974 |
| Rwork | 0.195 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 2.950 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.340 |
| Number of reflections | 14589 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 97.4 | 97.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-26% PEG4000, 100 mM HEPES, 200 mM lithium chloride, pH 6.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
