4I2F
Binary complex of mouse TdT with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28149 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.166, 84.428, 114.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.610 - 2.100 |
| R-factor | 0.1739 |
| Rwork | 0.173 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.116 | 0.727 |
| Number of reflections | 27387 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-26% PEG4000, 100 mM HEPES, 200 mM ammonium sulfate, pH 6.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
