4I20
Crystal structure of monomeric (V948R) primary oncogenic mutant L858R EGFR kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2011-06-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.044, 108.044, 103.095 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.570 - 3.340 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data scaling software | autoPROC |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.100 | 3.520 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.117 | 0.456 |
| Number of reflections | 10424 | |
| <I/σ(I)> | 17.6 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2.8M Sodium acetate trihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
