4I1S

Melanoma differentiation associated protein-5 Helicase domain complex with inhibitor Non-structural protein V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	51.070, 52.490, 120.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.141 - 2.293
R-factor	0.1817
Rwork	0.179
R-free	0.22960
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.173
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.293	2.290
<I/σ(I)>	13.64	2.58
Completeness [%]	99.2	83.8
Redundancy	7.17	6.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100mM HEPES pH7.5 18% (w/v) PEG1500 trace amounts of trypsin , VAPOR DIFFUSION, HANGING DROP, temperature 298K