4I18

Crystal structure of human prolactin receptor complexed with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.1271
Spacegroup name	P 61
Unit cell lengths	285.826, 285.826, 62.248
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.507 - 3.238
R-factor	0.1939
Rwork	0.191
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2R8S AND 3D48
RMSD bond length	0.016
RMSD bond angle	2.138
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	250.000	50.000	3.370
High resolution limit [Å]	3.238	7.000	3.238
Rmerge	0.207	0.059	0.750
Number of reflections	47509
<I/σ(I)>	5.6
Completeness [%]	99.4	98.9	97.8
Redundancy	6.5	7.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	160 mM calcium acetate, 80 mM sodium cacodylate, pH 6.2, 11% PEG8000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K