4I18
Crystal structure of human prolactin receptor complexed with Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 61 |
| Unit cell lengths | 285.826, 285.826, 62.248 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.507 - 3.238 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2R8S AND 3D48 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 250.000 | 50.000 | 3.370 |
| High resolution limit [Å] | 3.238 | 7.000 | 3.238 |
| Rmerge | 0.207 | 0.059 | 0.750 |
| Number of reflections | 47509 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 99.4 | 98.9 | 97.8 |
| Redundancy | 6.5 | 7.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 160 mM calcium acetate, 80 mM sodium cacodylate, pH 6.2, 11% PEG8000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
