4I16
Crystal structure of CARMA1 CARD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-05-04 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 82.285, 82.285, 82.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.992 - 1.751 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.992 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 16403 | |
| Completeness [%] | 88.6 | 64.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | 1.6M MgSO4, 0.1M MES, pH 6.5, LIQUID DIFFUSION, temperature 293K |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | 1.6M MgSO4, 0.1M MES, pH 6.5, LIQUID DIFFUSION, temperature 293K |
