4I0Y
CRYSTAL STRUCTURE OF RABBIT RYANODINE RECEPTOR 1 (RESIDUES 1-536) DISEASE MUTANT C36R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 170.381, 170.381, 302.113 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.180 - 2.800 |
| R-factor | 0.24284 |
| Rwork | 0.243 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.180 | 4.430 | 2.950 |
| High resolution limit [Å] | 2.800 | 3.960 | 2.800 |
| Number of reflections | 41758 | ||
| <I/σ(I)> | 18 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 11.4 | 11.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9.5 | pH 9.5, EVAPORATION |
