4I0Y

CRYSTAL STRUCTURE OF RABBIT RYANODINE RECEPTOR 1 (RESIDUES 1-536) DISEASE MUTANT C36R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2010-06-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	170.381, 170.381, 302.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.180 - 2.800
R-factor	0.24284
Rwork	0.243
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.975
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.180	4.430	2.950
High resolution limit [Å]	2.800	3.960	2.800
Number of reflections	41758
<I/σ(I)>	18
Completeness [%]	100.0	100	100
Redundancy	11.4	11.4	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9.5		pH 9.5, EVAPORATION