4I0N
Pore forming protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.444, 29.698, 56.265 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 26.400 - 1.800 |
| R-factor | 0.17016 |
| Rwork | 0.168 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.400 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 25514 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M Bis-Tris propane, 0.8M LiSO4, 5%(v/v) glycerol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
