4I0A
structure of the mutant Catabolite gene activator protein V132A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Detector technology | CCD |
Collection date | 2011-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9687 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.900, 93.300, 104.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.490 - 2.200 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.23317 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.021 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.500 | 2.257 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 22117 | |
Completeness [%] | 99.5 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-10% Peg3350, 15-20% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |