4HZV

The crystal structure of influenza A neuraminidase N3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	I 4
Unit cell lengths	106.144, 106.144, 64.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.566 - 1.800
R-factor	0.1491
Rwork	0.147
R-free	0.18550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bat
RMSD bond length	0.005
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	32203
Completeness [%]	99.2	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	20mM Tris, pH 8.0, 50mM NaCl, 0.1M BIS-TRIS propane (pH 9.0), 10% v/v Jeffamine ED-2001 (pH 7.0), VAPOR DIFFUSION, temperature 291K