4HZD

Crystal structure of Serine acetyltransferase in complex with Coenzyme A from Brucella abortus strain S19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-04-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5417
Spacegroup name	H 3
Unit cell lengths	104.347, 104.347, 105.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.680 - 1.870
R-factor	0.1852
Rwork	0.183
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.598
Data reduction software	AUTOMAR
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.918
High resolution limit [Å]	1.870	1.870
Rmerge	0.100
Number of reflections	35422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	5% PEG 1000, 5% PEG 3350, 5% MPD, 0.1 M Ethylene glycol, Tris 100 mM, 20-40 mM MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K