4HZB

Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.9790
Spacegroup name	P 21 21 2
Unit cell lengths	56.199, 126.943, 143.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.054 - 2.600
R-factor	0.2159
Rwork	0.216
R-free	0.27060
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.105	0.493
Number of reflections	32299
<I/σ(I)>	24.9	2.8
Completeness [%]	99.8	96
Redundancy	13.9	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.01M ferric chloride hexahydrate, 0.1M tri-sodium citrate dihydrate pH5.6, 10% v/v jeffamine M-600 , pH 5.6 , VAPOR DIFFUSION, SITTING DROP, temperature 277 K