4HZ9

Crystal structure of the type VI native effector-immunity complex Tae3-Tai3 from Ralstonia pickettii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	56.166, 144.382, 129.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.286 - 2.400
R-factor	0.1933
Rwork	0.191
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hzb
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.532
Number of reflections	20842
<I/σ(I)>	18.4	2
Completeness [%]	99.8	90.4
Redundancy	7	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.01M ferric chloride hexahydrate, 0.1M tri-sodium citrate dihydrate pH5.6, 10% v/v jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 277K