4HZ1

Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	77
Detector technology	CCD
Collection date	2012-03-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.608
Spacegroup name	P 21 21 21
Unit cell lengths	47.309, 98.033, 108.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 2.200
Rwork	0.245
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	43.000
High resolution limit [Å]	2.200
Number of reflections	26498
<I/σ(I)>	13.48
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	3.0 - 3.5 M ammonium sulfate, 0.5 M lithium nitrate, 0.1 M sodium acetate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K