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4HYY

Filament of octameric rings of DMC1 recombinase from Homo sapiens

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08ID-1
Synchrotron siteCLSI
Beamline08ID-1
Temperature [K]100
Detector technologyCCD
Collection date2010-09-24
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.98
Spacegroup nameP 4
Unit cell lengths123.997, 123.997, 91.713
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.450 - 2.603
R-factor0.18187
Rwork0.179
R-free0.24615
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1v5w
RMSD bond length0.014
RMSD bond angle1.906
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.6.0117)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]47.5002.690
High resolution limit [Å]2.6002.600
Rmerge0.0680.419
Number of reflections42118
<I/σ(I)>25.72.7
Completeness [%]97.082.1
Redundancy7.16
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.42946% PEG 3350, 0.5 M NaCl, 0.05 M MgCl2, 0.05 M HEPES-NACl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K

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