4HYY
Filament of octameric rings of DMC1 recombinase from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 4 |
| Unit cell lengths | 123.997, 123.997, 91.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 2.603 |
| R-factor | 0.18187 |
| Rwork | 0.179 |
| R-free | 0.24615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v5w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.906 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.068 | 0.419 |
| Number of reflections | 42118 | |
| <I/σ(I)> | 25.7 | 2.7 |
| Completeness [%] | 97.0 | 82.1 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 6% PEG 3350, 0.5 M NaCl, 0.05 M MgCl2, 0.05 M HEPES-NACl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
