4HYT
Na,K-ATPase in the E2P state with bound ouabain and Mg2+ in the cation-binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.470, 118.080, 494.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.829 - 3.404 |
| R-factor | 0.2265 |
| Rwork | 0.226 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb ID 3N23 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 72261 | |
| <I/σ(I)> | 15.96 | 1.41 |
| Completeness [%] | 75.7 | 4.8 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.2 | 292 | 16-17% PEG 2000 mme, 10% Glycerol, 200 mM MgCl2, 100 mM MES-NMDG pH 6.2, 100 mM Urea, 5% tert-Butanol, 5mM DTT, 1.6 mM Sucrose monodecanoate, hanging drop, temperature 292K |
