4HYD

Structure of a presenilin family intramembrane aspartate protease in C2221 space group

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.07171, 1.05384, 1.07206
Spacegroup name	C 2 2 21
Unit cell lengths	114.077, 170.025, 181.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.577 - 3.800
R-factor	0.3073
Rwork	0.307
R-free	0.30810
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.663
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.800
Number of reflections	15330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.6	291	0.1M Glycine, 0.2M (NH4)2SO4, 20%(w/v) PEG500MME, 6%(w/v) Glycerol, 0.04%(w/v) Anapoe-C12E8, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K