4HY7

Structural and biochemical characterization of a cytosolic wheat cyclophilin TaCypA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Temperature [K]	197
Detector technology	CCD
Collection date	2012-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9733
Spacegroup name	P 21 21 21
Unit cell lengths	37.914, 49.847, 78.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.350 - 1.200
R-factor	0.18732
Rwork	0.187
R-free	0.20439
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e1q
RMSD bond length	0.027
RMSD bond angle	2.305
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	1.270	1.220
High resolution limit [Å]	1.200	1.240	1.200
Number of reflections	47120
Completeness [%]	99.0	99.4	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG 8000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K