4HY2
Crystal structure of Plk1 Polo-box domain in complex with PL-42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.425, 50.262, 57.456 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.199 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.300 | 50.262 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.028 | 0.249 | |
| Total number of observations | 1214 | 5663 | |
| Number of reflections | 13200 | ||
| <I/σ(I)> | 13.6 | 20.2 | 3 |
| Completeness [%] | 98.1 | 95 | 97.8 |
| Redundancy | 3 | 2.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10 | 293 | 0.1M CAPS pH 8, 0.2M LiSO4, 0.8M K2HPO4 NaH2PO4, vapor diffusion, temperature 293K |
