4HXV
Crystal structure of 3'(2'),5'-bisphosphate nucleotidase1 from Entamoeba histolytica in complex with AMP and metal ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.618, 63.877, 117.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.310 - 2.600 |
| R-factor | 0.2326 |
| Rwork | 0.230 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDG |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.185 | 0.430 |
| Number of reflections | 9190 | |
| <I/σ(I)> | 5.319 | |
| Completeness [%] | 98.9 | 88.8 |
| Redundancy | 11.6 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 28% PEG4000, 200mM NaCl, 5mM MgCl2, 5mM adenosine monophosphate (AMP), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
