4HXF
Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 184.057, 184.057, 145.529 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 1.601 |
| R-factor | 0.15617 |
| Rwork | 0.155 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hxe |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.248 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.066 | 0.738 |
| Number of reflections | 122438 | |
| <I/σ(I)> | 22.25 | 2.57 |
| Completeness [%] | 99.2 | 94.4 |
| Redundancy | 7.03 | 5.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.5 M 1,6-hexanediol, 0.20 M MgCl2, 0.1 M Tris/HCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
