4HXA
Crystal structure of 13D9 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-19 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.750, 100.280, 132.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.060 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAB FROM YST9.1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.985 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.058 | 0.293 |
| Number of reflections | 31114 | |
| <I/σ(I)> | 12 | 3.8 |
| Completeness [%] | 88.4 | 91.8 |
| Redundancy | 4.67 | 4.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 10000, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
