4HX8
Structure of metal-free MNTR mutant E11K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.920, 86.710, 91.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.355 - 2.001 |
| R-factor | 0.2386 |
| Rwork | 0.235 |
| R-free | 0.29940 |
| Structure solution method | EPMR |
| Starting model (for MR) | 2hyf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.006 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.277 | |
| Number of reflections | 21377 | |
| <I/σ(I)> | 17.4 | 5.7 |
| Completeness [%] | 99.2 | 94.5 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5% PEG 4000, 4% ethanol, 2% 1,4-butanediol, 4 mM EDTA, 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
