4HX7
Structure of MNTR E11K mutant complexed with Cd2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.119, 46.252, 74.142 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 74.000 - 1.900 |
| R-factor | 0.2459 |
| Rwork | 0.244 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.093 | 0.357 |
| Number of reflections | 25216 | |
| <I/σ(I)> | 6.4 | 2.5 |
| Completeness [%] | 95.4 | 78.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40% ETHYLENE GLYCOL, 10% PEG 3000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
