4HWN

Crystal structure of the second Ig-C2 domain of human Fc-receptor like A (FCRLA), Isoform 9 [NYSGRC-005836]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 21 21 21
Unit cell lengths	31.198, 35.517, 73.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.941 - 2.006
R-factor	0.1863
Rwork	0.184
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rjd
RMSD bond length	0.007
RMSD bond angle	1.049
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.070	0.059	0.213
Number of reflections	5840
<I/σ(I)>	31.1
Completeness [%]	99.0	98	100
Redundancy	10.6	9.4	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol; Reservoir (0.1M Sodium Cacodylate pH 6.5, 0.2M Sodium Chloride, 2M Ammonium Sulfate); Cryoprotection (Reservoir + 2M Lithium Sulfate), Sitting Drop Vapor Diffusion, temperature 298K, VAPOR DIFFUSION, SITTING DROP