4HWI

Crystal structure of ATBAG1 in complex with HSP70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	61.928, 87.360, 65.070
Unit cell angles	90.00, 109.18, 90.00

Refinement procedure

Resolution	35.604 - 2.273
R-factor	0.2055
Rwork	0.203
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1HX1 AND 4HWC
RMSD bond length	0.007
RMSD bond angle	1.008
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.6.4_486)
Refinement software	PHENIX ((PHENIX.REFINE: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.604	2.340
High resolution limit [Å]	2.273	2.273
Number of reflections	29495
Completeness [%]	99.2	90.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	0.02 M citric acid / 0.08 M Bis-Tris propane, pH 8.8, 20% PEG3350, VAPOR DIFFUSION, temperature 277K