4HWA
Crystal Structure of Escherichia coli MscS Wildtype (Open State)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 128.240, 149.970, 175.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.914 - 4.370 |
| R-factor | 0.268 |
| Rwork | 0.267 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.576 | 4.480 |
| High resolution limit [Å] | 4.370 | 4.370 |
| Rmerge | 0.597 | |
| Number of reflections | 21374 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 95.0 | 99.8 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris HCl pH 8.5, 30% PEG 400, 200 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
