4HV6
Structure of MNTR H77A mutant in apo- and mn-bound forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.734, 51.734, 175.564 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.800 - 2.300 |
| Rwork | 0.252 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.800 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.081 |
| Number of reflections | 12767 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% ETHOXYETHANOL,4% ETHYLENE GLYCOL, 90 MM MGCL2, 60 MM TRIS HCL, 1 MM MNSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
