4HV2

Laser-induced microfragmentation of lysozyme crystals allows X-ray nanodiffraction characterization of individual domains (lb5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Detector technology	CCD
Collection date	2007-12-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	79.703, 79.703, 37.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.359 - 2.501
R-factor	0.2053
Rwork	0.200
R-free	0.31860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.519
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.359	39.852	1.610
High resolution limit [Å]	1.525	4.820	1.525
Rmerge		0.026	0.026
Total number of observations		2374	4648
Number of reflections	3371
<I/σ(I)>	3.1	6.1	0.3
Completeness [%]	78.9	71.2	65.1
Redundancy	4.6	4.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	LB-nanofilms were generated by the Langmuir Blodgett (LB) technique and its variation, a modified Langmuir Schaeffer technique (LS). 40 mg/ml, 50 mM, sodium acetate pH 4.5, 0.9 NaCl 1:1 in 50 mM sodium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 298K