4HUH
Structure of the bacteriophage T4 tail terminator protein, gp15 (C-terminal truncation mutant 1-261).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.874, 76.305, 93.893 |
| Unit cell angles | 109.04, 104.62, 89.30 |
Refinement procedure
| Resolution | 45.911 - 3.200 |
| R-factor | 0.1727 |
| Rwork | 0.171 |
| R-free | 0.20210 |
| Structure solution method | SIRAS (using Pt derivative) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.911 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.077 | 0.210 |
| Number of reflections | 25229 | |
| <I/σ(I)> | 18.7 | 5.9 |
| Completeness [%] | 91.7 | 72 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 20% w/v Polyethylene glycol 3,350, 0.2 M Ammonium formate., pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
