4HUC
Crystal structure of LdtMt2, a L,D-transpeptidase from Mycobacterium tuberculosis: domain B and C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 116.027, 121.430, 123.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 1.860 |
| R-factor | 0.16624 |
| Rwork | 0.165 |
| R-free | 0.18772 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.619 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.500 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.065 | 0.065 |
| Number of reflections | 71548 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.1 | 87.6 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3 M NaOAc pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
