4HU6
Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Cyclic product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-22 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.945, 33.102, 41.075 |
| Unit cell angles | 93.91, 111.95, 98.74 |
Refinement procedure
| Resolution | 28.520 - 2.300 |
| R-factor | 0.2603 |
| Rwork | 0.259 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2ZTA |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.479 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.520 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.050 |
| Number of reflections | 6255 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 95.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate pH 4.6, 1.6 M sodium chloride, 5% w/v PEG 1500; crystal was treated with 1 equiv. sodium periodate relative to protein and allowed to incubate for 2 days prior to harvesting, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
