4HU5
Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Linear precursor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-12 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.729, 30.907, 33.306 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.2064 |
| Rwork | 0.202 |
| R-free | 0.26809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2ZTA |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.106 |
| Number of reflections | 3576 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 99.7 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate pH 4.6, 1.6 M sodium chloride, 5% w/v PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
