4HU4
Crystal structure of EAL domain of the E. coli DosP - dimeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.600, 171.170, 48.830 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 19.992 - 2.400 |
| R-factor | 0.2368 |
| Rwork | 0.233 |
| R-free | 0.28010 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 28963 | |
| Completeness [%] | 99.1 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20 % PEG 6000, 0.2 M calcium chloride, 0.1 M HEPES pH 7.0, vapor diffusion, hanging drop, temperature 293K |
