4HU3
Crystal structure of EAL domain of the E. coli DosP - monomeric form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97139, 0.97896, 0.97139 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 139.810, 139.810, 139.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.366 - 3.301 |
| R-factor | 0.2667 |
| Rwork | 0.264 |
| R-free | 0.32310 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.660 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 13227 | |
| Completeness [%] | 99.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 0.5 M potassium sodium tartrate, 0.1 M MES pH 6.3, vapor diffusion, hanging drop, temperature 293K |
