4HU3

Crystal structure of EAL domain of the E. coli DosP - monomeric form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97139, 0.97896, 0.97139
Spacegroup name	I 2 3
Unit cell lengths	139.810, 139.810, 139.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.366 - 3.301
R-factor	0.2667
Rwork	0.264
R-free	0.32310
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.400
High resolution limit [Å]	3.300	3.300
Number of reflections	13227
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	0.5 M potassium sodium tartrate, 0.1 M MES pH 6.3, vapor diffusion, hanging drop, temperature 293K