4HTW
SIVmac239 capsid N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.614, 56.663, 102.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.273 - 2.900 |
| R-factor | 0.2558 |
| Rwork | 0.254 |
| R-free | 0.29270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
| Rmerge | 0.063 | 0.036 | 0.365 |
| Number of reflections | 3537 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.9 | 100 | 98.8 |
| Redundancy | 4.4 | 3.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 10% PEG 2000 MME, 10mM nickel chloride and 100mM Tris., pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
