4HTV

Mouse importin alpha: BFDV Cap NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.200, 89.900, 99.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 3.000
R-factor	0.17558
Rwork	0.173
R-free	0.22804
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.848
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.990	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.153
Number of reflections	16074
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.675 M trisodium citrate, 20 mM DTT, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K