4HTT

Crystal Structure of Twin Arginine Translocase Receptor- TatC in DDM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.08
Spacegroup name	I 41 2 2
Unit cell lengths	142.015, 142.015, 251.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 6.800
R-factor	0.3437
Rwork	0.344
R-free	0.41820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.874	39.250	7.170
High resolution limit [Å]	6.800	21.500	6.800
Rmerge		0.026	0.790
Total number of observations		390	2286
Number of reflections	2281
<I/σ(I)>	22.5	17.8	0.9
Completeness [%]	95.9	77.8	96.9
Redundancy	6.8	5.5	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	35% (v/v) PEG 400, ADA pH 6.6 and 0.1 M Potassium phosphate monobasic., VAPOR DIFFUSION, SITTING DROP, temperature 293K