4HTL

Lmo2764 protein, a putative N-acetylmannosamine kinase, from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-04
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	F 2 2 2
Unit cell lengths	87.694, 121.989, 153.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 1.640
R-factor	0.1298
Rwork	0.128
R-free	0.16860
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.900	50.000	1.670
High resolution limit [Å]	1.640	4.450	1.640
Rmerge	0.087	0.065	0.605
Number of reflections	49631
<I/σ(I)>	16.2		2.1
Completeness [%]	99.4	98.2	96.7
Redundancy	7.4	7.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2 M lithium sulfate, 0.1 M Tris-HCl, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K