4HT5
CO2 concentrating mechanism protein P, CcmP form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.350, 137.355, 152.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.265 - 2.510 |
| R-factor | 0.2572 |
| Rwork | 0.257 |
| R-free | 0.29080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (autoMR) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.864 | 2.570 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.256 | 1.122 |
| Number of reflections | 86082 | |
| <I/σ(I)> | 1.411 | |
| Completeness [%] | 97.3 | 86.9 |
| Redundancy | 6.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 100 mM sodium acetate, 1.2 M sodium formate, and 20% PEG 400 , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
