4HSS
Structure of the Full-Length Major Pilin SpaD from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.917000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.828, 56.791, 435.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 2.500 |
| R-factor | 0.1904 |
| Rwork | 0.187 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hsq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.060 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.695 | |
| Number of reflections | 27956 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 95.2 | 96.6 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 15% PEG 600, 0.2M imidazole-malate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
