4HRY

The structure of Arabidopsis thaliana KAI2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95390
Spacegroup name	P 21 21 21
Unit cell lengths	63.560, 66.260, 78.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.960 - 1.500
R-factor	0.1774
Rwork	0.176
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.505
High resolution limit [Å]	1.500
Number of reflections	51205
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	1.4M sodium potassium phosphate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K